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Improved Masson Trichrome Stain on Plastic-Embedded Tissue

Danny Smith

Open publisher page 3 citations

Abstract

A modified Masson's trichrome method for use on plastic-embedded tissues is presented. The trichrome stain can be vastly improved by extending the iron hematoxylin, Biebrich scarlet-acid fuchsin, and aniline blue staining times. After rinsing in acidified water the blue-colored background can be removed by dipping in 2-butoxyethanol. This method gives good results on tissues that are embedded in glycol-methacrylate plastics which are not removed prior to staining. The effects are similar to those obtained with routine, paraffin-embedded tissue.

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What this paper is about

A modified Masson's trichrome method for use on plastic-embedded tissues is presented. The trichrome stain can be vastly improved by extending the iron hematoxylin, Biebrich scarlet-acid fuchsin, and aniline blue staining times. After rinsing in acidified water the blue-colored background can be removed by dipping in 2-butoxyethanol. This method gives good results on tissues that are embedded in glycol-methacrylate plastics which are not removed prior to staining. The effects are similar to those obtained with routine, paraffin-embedded tissue.

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Available abstract

A modified Masson's trichrome method for use on plastic-embedded tissues is presented. The trichrome stain can be vastly improved by extending the iron hematoxylin, Biebrich scarlet-acid fuchsin, and aniline blue staining times. After rinsing in acidified water the blue-colored background can be removed by dipping in 2-butoxyethanol. This method gives good results on tissues that are embedded in glycol-methacrylate plastics which are not removed prior to staining. The effects are similar to those obtained with routine, paraffin-embedded tissue.

Key concepts: Trichrome, Masson's trichrome stain, Trichrome stain, Staining, Stain, H&E stain, Chemistry, Pathology

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