Variations in Expression and Release of Botulinum Neurotoxin in Clostridium botulinum Type A Strains

In this study, we characterized the production and release of botulinum neurotoxin during growth of two Clostridium botulinum strains, Hall A and NCTC2916, of distinct gene organizations. The intra- and extracellular fractions of the bacterial cells harvested at various stages of growth were analyzed for the neurotoxin. Both strains exhibited a temporal neurotoxin gene expression; however, these two strains differ in their patterns of growth, toxin production, toxin release, and post-translational nicking. Comparing to the NCTC2916 strain, the Hall A strain showed an extended stationary phase, delayed autolysis, and earlier expression and release of neurotoxin. Understanding the differences between these two toxin-producing strains may provide insights into the toxinogenesis of C. botulinum.