1999•Journal of Veterinary Medical ScienceOpen access

Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Tetsuya Yoshida, Michiko Takeuchi, Moritoshi Sato, Katsuya Hirai

Abstract

Twenty epidemiologically unrelated Listeria monocytogenes strains isolated from different animals, locations and on different dates in Japan were classified into 18 types by the random amplified polymorphic DNA (RAPD) fingerprinting technique with four primers. Further, seven epidemiologically related L. monocytogenes strains isolated from raw milk and a bulk tank on a dairy farm represented the same RAPD type suggesting that they were all of the same origin. Therefore, RAPD-polymerase chain reaction (PCR) analysis, which is rapid, simple and inexpensive to perform, can be used in surveys as a convenient epidemiological technique.

Open-access reader

About this research paper

What this paper is about

Why it matters

OpenAlex reports 21 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: RAPD, Listeria monocytogenes, Typing, Biology, DNA profiling, Polymerase chain reaction, Raw milk, DNA