2013•Journal of Clinical MicrobiologyOpen access

Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Pulsed-Field Gel Electrophoresis Typing of Acinetobacter baumannii in China

Yuan Hu, Boqing Li, Dazhi Jin, Zhigang Cui, Xiaoxia Tao, Binghua Zhang, Jianzhong Zhang

Abstract

A panel of seven variable-number tandem-repeat (VNTR) markers was selected for Acinetobacter baumannii typing analysis (MLVA-7). Compared with pulsed-field gel electrophoresis (PFGE), MLVA-7 provided greater discrimination. We modified the criteria for MLVA complex assignments proposed previously, and a remarkable congruence between MLVA-7- and PFGE-based strain clustering was observed.

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Key concepts: Multiple Loci VNTR Analysis, Variable number tandem repeat, Pulsed-field gel electrophoresis, Acinetobacter baumannii, Typing, Biology, DNA profiling, Genetics