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1982•Planta MedicaRequires access

Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents

B. Meyer, N. R. Ferrigni, J. E. Putnam, Linda B. Jacobsen, David E. Nichols, J. McLaughlin

Open publisher page 4,836 citations

Abstract

A method, utilizing brine shrimp ( Artemia salina L EACH ), is proposed as a simple bioassay for natural product research. The procedure determines LC 50 values in µg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool. Key Word Index Artemia salina - Brine shrimp - Bioassay methods - Euphorbiaceae seeds - 9KB and 9PS cytotoxicities

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What this paper is about

A method, utilizing brine shrimp ( Artemia salina L EACH ), is proposed as a simple bioassay for natural product research. The procedure determines LC 50 values in µg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool. Key Word Index Artemia salina - Brine shrimp - Bioassay methods - Euphorbiaceae seeds - 9KB and 9PS cytotoxicities

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Available abstract

A method, utilizing brine shrimp ( Artemia salina L EACH ), is proposed as a simple bioassay for natural product research. The procedure determines LC 50 values in µg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool. Key Word Index Artemia salina - Brine shrimp - Bioassay methods - Euphorbiaceae seeds - 9KB and 9PS cytotoxicities

Key concepts: Brine shrimp, Bioassay, Artemia salina, Shrimp, Brine, Chromatography, Biology, Natural Product Research

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