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Analysis of polymeric proteins by two‐dimensional polyacrylamide gel electrophoresis on one slab in the presence and absence of sodium dodecyl sulfate

H. Bloemendal, Kees Jansen

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Abstract

Abstract The method presented was developed specifically to be used for the electrophoretic analysis of polymeric proteins in order to establish the number of aggregates and that of their subunits. Electrophoresis is carried out on a single slab; without sodium dodecyl sulfate (SDS) in the first dimension and with SDS in the second dimension. The method is possible since for one‐dimensional SDS gel electrophoresis, SDS is only required in the cathode vessel.

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What this paper is about

Abstract The method presented was developed specifically to be used for the electrophoretic analysis of polymeric proteins in order to establish the number of aggregates and that of their subunits. Electrophoresis is carried out on a single slab; without sodium dodecyl sulfate (SDS) in the first dimension and with SDS in the second dimension. The method is possible since for one‐dimensional SDS gel electrophoresis, SDS is only required in the cathode vessel.

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Available abstract

Abstract The method presented was developed specifically to be used for the electrophoretic analysis of polymeric proteins in order to establish the number of aggregates and that of their subunits. Electrophoresis is carried out on a single slab; without sodium dodecyl sulfate (SDS) in the first dimension and with SDS in the second dimension. The method is possible since for one‐dimensional SDS gel electrophoresis, SDS is only required in the cathode vessel.

Key concepts: Sodium dodecyl sulfate, Electrophoresis, Gel electrophoresis, Chromatography, Chemistry, Polyacrylamide gel electrophoresis, Gel electrophoresis of proteins, Sodium

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Analysis of polymeric proteins by two‐dimensional polyacrylamide gel electrophoresis on one slab in the presence and absence of sodium dodecyl sulfate — Research Paper | ScholarLens