1987•Agricultural and Biological ChemistryOpen access

Expression of amplified cDNA sequences encoding human interleukin 2 in human cells.

Kazuya Onomichi, Yuzuru Eto, Hiroshiro Shibai

Abstract

Expression of human interleukin 2 (IL-2) at high levels has been achieved in human cells by cotransfection and subsequent coamplification of the transfected sequences.IL-2 production by these cells reached 2700 U/ml of culture medium.This productivity was higher than the lOO U/ml

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Key concepts: Complementary DNA, Molecular biology, Transfection, Plasmid, HeLa, Dihydrofolate reductase, Jurkat cells, Cell culture