2009•Acta Pathologica Microbiologica Scandinavica Section C ImmunologyRequires access
COMPARISON BETWEEN LUMINOL- AND LUCIGENINDEPENDENT CHEMILUMINESCENCE OF POLYMORPHONUCLEAR LEUKOCYTES
H. ANANSSON, Olle Stendahl, Cláes Dahlgren
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Abstract
Phorbolmyristate acetate (PMA) induced both a luminol- and a lucigenindependent chemiluminescence response in normal polymorphonuclear leukocytes (PMNL). PMNL isolated from a patient with a myeloperoxidase (MPO)-deficiency were found to produce almost no luminol-dependent chemiluminescence. The lucigenin-dependent chemiluminescence response was, however, found not to differ between normal and MPO-deficient cells. From these results it was concluded that the luminol- and the lucigenin-dependent chemiluminescence differed in the dependence of MPO. Results obtained with cell-free MPO-H2O2- and superoxide anion producing systems further supported this conclusion.