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2010•FEBS LettersRequires access

Site‐specific cleavage of the 40S ribosomal subunit reveals eukaryote‐specific ribosomal protein S28 in the subunit head

Alexey A. Malygin, Г. Г. Карпова

Open publisher page 6 citations

Abstract

After resolving the crystal structure of the prokaryotic ribosome, mapping the proteins in the eukaryotic ribosome is a challenging task. We applied RNase H digestion to split the human 40S ribosomal subunit into head and body parts. Mass spectrometry of the proteins in the 40S subunit head revealed the presence of eukaryote-specific ribosomal protein S28e. Recombinant S28e was capable of specific binding to the 3' major domain of the 18S rRNA (K(a)=8.0±0.5×10(9) M(-1)). We conclude that S28e has a binding site on the 18S rRNA within the 40S subunit head.

About this research paper

What this paper is about

After resolving the crystal structure of the prokaryotic ribosome, mapping the proteins in the eukaryotic ribosome is a challenging task. We applied RNase H digestion to split the human 40S ribosomal subunit into head and body parts. Mass spectrometry of the proteins in the 40S subunit head revealed the presence of eukaryote-specific ribosomal protein S28e. Recombinant S28e was capable of specific binding to the 3' major domain of the 18S rRNA (K(a)=8.0±0.5×10(9) M(-1)). We conclude that S28e has a binding site on the 18S rRNA within the 40S subunit head.

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OpenAlex reports 6 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

After resolving the crystal structure of the prokaryotic ribosome, mapping the proteins in the eukaryotic ribosome is a challenging task. We applied RNase H digestion to split the human 40S ribosomal subunit into head and body parts. Mass spectrometry of the proteins in the 40S subunit head revealed the presence of eukaryote-specific ribosomal protein S28e. Recombinant S28e was capable of specific binding to the 3' major domain of the 18S rRNA (K(a)=8.0±0.5×10(9) M(-1)). We conclude that S28e has a binding site on the 18S rRNA within the 40S subunit head.

Key concepts: Eukaryotic Small Ribosomal Subunit, Protein subunit, Eukaryotic Large Ribosomal Subunit, Ribosomal protein, Eukaryote, Ribosomal RNA, Cleavage (geology), Biology

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Site‐specific cleavage of the 40S ribosomal subunit reveals eukaryote‐specific ribosomal protein S28 in the subunit head — Research Paper | ScholarLens