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1994•Journal of Applied Animal ResearchRequires access

Restriction Map of Cloned 3.8 kb PstI Fragment of Bovine Herpes Virus-1 DNA

Praveen Kumar Gupta, Anant Rai

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Abstract

Gupta, P.K. and Rai, A. 1994. Restriction map of cloned 3.8 kb PstI fragment of bovine herpes virus-1 DNA. J. Appl. Anim. Res., 6: 143–149. A 3.8 kb Pstl fragment of bovine herpesvirus-1 DNA was selectively cloned into pUC9 plasmid vector. The recombinant plasmid with the insert was analyzed using restriction endonucleases. On the basis of restriction digested fragment pattern, partial restriction site map was prepared for a few restriction endonucleases. Shot gun cloning of PstI digested DNA fragments yielded two clones having double inserts.

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What this paper is about

Gupta, P.K. and Rai, A. 1994. Restriction map of cloned 3.8 kb PstI fragment of bovine herpes virus-1 DNA. J. Appl. Anim. Res., 6: 143–149. A 3.8 kb Pstl fragment of bovine herpesvirus-1 DNA was selectively cloned into pUC9 plasmid vector. The recombinant plasmid with the insert was analyzed using restriction endonucleases. On the basis of restriction digested fragment pattern, partial restriction site map was prepared for a few restriction endonucleases. Shot gun cloning of PstI digested DNA fragments yielded two clones having double inserts.

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Available abstract

Gupta, P.K. and Rai, A. 1994. Restriction map of cloned 3.8 kb PstI fragment of bovine herpes virus-1 DNA. J. Appl. Anim. Res., 6: 143–149. A 3.8 kb Pstl fragment of bovine herpesvirus-1 DNA was selectively cloned into pUC9 plasmid vector. The recombinant plasmid with the insert was analyzed using restriction endonucleases. On the basis of restriction digested fragment pattern, partial restriction site map was prepared for a few restriction endonucleases. Shot gun cloning of PstI digested DNA fragments yielded two clones having double inserts.

Key concepts: PstI, Restriction enzyme, Plasmid, Insert (composites), Recombinant DNA, Restriction site, Restriction fragment, Restriction map

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