2006•Journal of Clinical MicrobiologyRequires access

Evaluation of a Real-Time Nucleic Acid Sequence-Based Amplification Assay Using Molecular Beacons for Detection of Human Immunodeficiency Virus Type 1

Daniel R. McClernon, Cindy Vavro, Marty St. Clair

Abstract

We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.

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Key concepts: NASBA, Molecular beacon, Nucleic acid, Biology, Virology, Recombinant DNA, Human immunodeficiency virus (HIV), Virus