Infection of Norway spruce by Armillaria under controlled conditions
U. Zollfrank, Bertold Hock
Abstract
U. Zollfrank, Bertold Hock
Abstract
Abstract A method for the infection of Norway spruce by Armillaria under defined conditions is described. The joint culture of seedlings and Armillaria rhizomorphs on a Spbagnum‐Perlite substrate under a controlled light‐dark and temperature regime guaranteed a high yield of infections, which became visible in the root system within 6 months. The localisation of the fungus was achieved by means of immunofluorescent techniques using anti‐Armillaria antibodies raised in rabbits.
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Abstract A method for the infection of Norway spruce by Armillaria under defined conditions is described. The joint culture of seedlings and Armillaria rhizomorphs on a Spbagnum‐Perlite substrate under a controlled light‐dark and temperature regime guaranteed a high yield of infections, which became visible in the root system within 6 months. The localisation of the fungus was achieved by means of immunofluorescent techniques using anti‐Armillaria antibodies raised in rabbits.
Key concepts: Armillaria, Perlite, Fungus, Armillaria mellea, Botany, Biology, Substrate (aquarium), Yield (engineering)