Transient‐State Kinetic Evidence Against a Channelled Transfer of Glyceraldehyde 3‐Phosphate from Aldolase to Glyceraldehyde‐3‐Phosphate Dehydrogenase

Theoretical and experimental evidence is presented which leads to a rejection of the proposal that the transient‐state kinetics of the coupled two‐enzyme reaction involving aldolase and glyceraldehyde‐3‐phosphate dehydrogenase are indicative of a channelled transfer of glyceraldehyde 3‐phosphate from the producing enzyme to the consuming one.