Genetic variability in foxtail millet, Setaria italica (L.) P. Beauv.: Identification and classification of lines with RAPD markers

Abstract The DNA of 37 lines of Setaria italica, representative of Eurasian diversity, were used for the detection of polymorphism using the random amplified polymorphic DNA (RAPD) method. Four 10‐mer primers, differing from each other by one or two G‐C inversions, were used. Each one gave specific RAPD products. Twenty‐five bands were polymorphic and allowed the identification of 33 different genotypes. A factorial analysis of correspondence was performed on the presenc‐absence data, through which three genetic groups could be identified. These genetic groups were closely related to the geographical origin of the different lines: one central European and two Asiatic groups (the first Asiatic lines originating in latitudes below 35°N and the second comprising the Asiatic lines originating in latitudes above 35° N). The lines originating from western Europe were very variable and were dispersed in the three genetic groups described previously.