Determination of Vitamin D In Multivitamin Preparations by HPLC
P. A. Lotfy, Howard C. Jordi, JohnG. Bruno
Abstract
P. A. Lotfy, Howard C. Jordi, JohnG. Bruno
Abstract
A selective extraction for vitamin D was achieved from oils, dry concentrates and multivitamins without the need for saponification. This was accomplished by utilizing SEP-PAK cartridges as an alternative to the pre-column clean-up required in the standard vitamin D assay. The final LC analysis was streamlined by using Radial Compression Technology in place of the conventional analytical LC column.
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A selective extraction for vitamin D was achieved from oils, dry concentrates and multivitamins without the need for saponification. This was accomplished by utilizing SEP-PAK cartridges as an alternative to the pre-column clean-up required in the standard vitamin D assay. The final LC analysis was streamlined by using Radial Compression Technology in place of the conventional analytical LC column.
Key concepts: Saponification, Multivitamin, Chromatography, Cartridge, Chemistry, Vitamin, Extraction (chemistry), High-performance liquid chromatography