1994•Journal of Liquid ChromatographyRequires access

A Sensitive and Fast Method for the Determination of Polyamines in Biological Samples. Benzoyl Chloride Pre-Column Derivatization High-Performance Liquid Chromatography

Ying-Hua Mei

Abstract

A sensitive, rapid pre-column derivatization HPLC procedure for determination of biologically important polyamines is described. Benzoylated putrescine, cadaverine, norspermidine, spermidine, norspermine and spermine were separated on reverse-phase column using 42:58 acetonitrile:water as mobile phase and detected by UV absorption at 198 nm. The detection limits were: 0.8 pmol for putrescine, 1 pmol for spermidine and 1.3 pmol for spermine. The method was successfully applied to the analysis of polyamines in small samples of unicellular organisms.

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Key concepts: Putrescine, Cadaverine, Spermidine, Chromatography, Derivatization, Spermine, Dansyl chloride, Chemistry