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Giemsa-banding pattern of chromosomes in Phaseolus vulgaris L.

Sima Bhattacharya

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Abstract

Giemsa-banding pattern of chromosomes following the BSG technique and trypsin treatment in Phaseolus vulgaris var. Tsanava Red 41 (2n=22) has been reported. Both diploid and polytene chromosomes have been dealt with. Except one metacentric pair all other chromosomes are showing either terminal or intercalary or both types of bands. One of the metacentric pairs shows centromeric band. One of the chromosomes bearing secondary constriction has been found heteromorphic. Percentage of the Giemsa-banding over the diploid complement has been calculated as 70%. This Giemsa-banding technique was found suitable for detection of the chromosomes in the karyotype which was not possible before.

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Giemsa-banding pattern of chromosomes following the BSG technique and trypsin treatment in Phaseolus vulgaris var. Tsanava Red 41 (2n=22) has been reported. Both diploid and polytene chromosomes have been dealt with. Except one metacentric pair all other chromosomes are showing either terminal or intercalary or both types of bands. One of the metacentric pairs shows centromeric band. One of the chromosomes bearing secondary constriction has been found heteromorphic. Percentage of the Giemsa-banding over the diploid complement has been calculated as 70%. This Giemsa-banding technique was found suitable for detection of the chromosomes in the karyotype which was not possible before.

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Available abstract

Giemsa-banding pattern of chromosomes following the BSG technique and trypsin treatment in Phaseolus vulgaris var. Tsanava Red 41 (2n=22) has been reported. Both diploid and polytene chromosomes have been dealt with. Except one metacentric pair all other chromosomes are showing either terminal or intercalary or both types of bands. One of the metacentric pairs shows centromeric band. One of the chromosomes bearing secondary constriction has been found heteromorphic. Percentage of the Giemsa-banding over the diploid complement has been calculated as 70%. This Giemsa-banding technique was found suitable for detection of the chromosomes in the karyotype which was not possible before.

Key concepts: Giemsa stain, Biology, Secondary constriction, Karyotype, Phaseolus, Ploidy, Polytene chromosome, G banding

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