1982•ScienceRequires access
Active Genes Are Sensitive to Deoxyribonuclease I During Metaphase
Bruria Gazit, Howard Cedar, Israela Lerer, Ruth Voss
Open publisher page 86 citations
Abstract
The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.