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2010•Journal of Fish BiologyRequires access

Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences

Anastasia Imsiridou, George Minos, A. Gakopoulou, Vassilios Katsares, Theofanis Karidas, George N. Katselis

Open publisher page 16 citations

Abstract

A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.

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What this paper is about

A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.

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Available abstract

A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.

Key concepts: Biology, Mitochondrial DNA, Haplotype, 16S ribosomal RNA, Genetics, Gene, DNA sequencing, DNA

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Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences — Research Paper | ScholarLens