ScholarLens Back to search
2008•Journal of Bacteriology and VirologyOpen access

Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA

Woo-Jin Jeon, Byung-Sik Chang, Mi-Ja Park, Eun‐Kyoung Lee, Hoo-Don Joo, Jun-Hun Kwon, Kang-Seuk Choi

Open full text 4 citations

Abstract

Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens.A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples.Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA.Detection limit of IBDV by DAS-ELISA was approximately 10 2.7 EID 50 /ml.The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types.The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes.Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.

Open-access reader

About this research paper

What this paper is about

Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens.A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples.Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA.Detection limit of IBDV by DAS-ELISA was approximately 10 2.7 EID 50 /ml.The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types.The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes.Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.

Why it matters

OpenAlex reports 4 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens.A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples.Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA.Detection limit of IBDV by DAS-ELISA was approximately 10 2.7 EID 50 /ml.The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types.The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes.Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.

Key concepts: Infectious bursal disease, Virology, Virus, Antibody, Monoclonal antibody, Immunosuppression, Biology, Virulence

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA — Research Paper | ScholarLens