2005•Journal of Immunoassay and ImmunochemistryRequires access

Isolation of α‐ and β‐Subunits of Peak‐I hCG and Generation of Highly Specific Polyclonal Antisera

Pramod Vishwanath Prasad, Shail K. Chaube, Tulsidas G. Shrivastav, G.L. Kumari, S. Duraiswami, K. Muralidhar

Abstract

Development of polyclonal antisera is still a choice in some hard-pressed budget laboratories. In the present study, an attempt was made to isolate alpha- and beta-subunits from peak-I hCG, generation of polyclonal antisera and their characterization. The anti-hCG-a antisera showed titres of 1: 8000 and anti-hCG-beta antisera 1: 16,000 at 50% binding to radiolabelled hCG in RIA. Studies on specificity using anti hCG-beta antiserum demonstrated no cross-reaction with several hormones tested in the present study, except for hCG-beta and hCG, thus eliciting a highly specific hCG-beta antiserum.

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Key concepts: Antiserum, Polyclonal antibodies, Prolactin, Molecular biology, Chemistry, Hormone, Antibody, Biology