2009•Biocontrol ScienceOpen access

Evaluation of a New Most-Probable-Number (MPN) Dilution Plate Method for the Enumeration of Escherichia coli in Water Samples

Hidemasa Kodaka, Mikako Saito, Hideaki Matsuoka

Abstract

The purpose of this study was to evaluate the most-probable-number dilution plate (MPN plate) method developed for the enumeration of Escherichia coil in water samples. Sterilized water was inoculated with E. coli ATCC 11775 to give between 2-1600 MPN/100 ml. The MPN was determined for both the MPN plate and 5-tube methods from the MPN table. The average of the natural logarithm (In) MPN with standard deviations in 95 samples was 4.26 +/- 1.48 by the 5-tube-method and 4.18 +/- 1.45 by the MPN plate method. The correlation coefficient was 0.96. These results were not significantly different according to the paired t-test (p > 0.05).

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Key concepts: Enumeration, Most probable number, Plate count, Dilution, Mathematics, Chromatography, Escherichia coli, Correlation coefficient