1997•ACTA HISTOCHEMICA ET CYTOCHEMICAOpen access

Ki-67 Antigen Retrieval in Formalin- or Ethanol-fixed, Paraffin-embedded Tissues: An Enhancement Method for Immunohistochemical Staining with Autoclave Treatment.

Yoshiro Tsuji, Katsuyuki Kusuzaki, Yasusuke Hirasawa, Massimo Serra, Nicola Baldini

Abstract

We developed a new immunohistochemical method to visualize Ki-67 antigen in formalin-fixed, paraffin-embedded sections of bone tumors. The method uses autoclave pretreatment of sections immersed in citrate buffer before incubation with the MIB 1 antibody, which reacts with the Ki-67 gene product. MIB 1 antibody showed strong and discrete staining of cell nuclei in proliferating tumor cells. This method also retrieved antigen in paraffin-embedded sections of alcohol-fixed tissue. Therefore, tissues previously considered unsuitable for immunohistochemical analysis can be studied after autoclave treatment.

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Available abstract

Key concepts: Immunohistochemistry, Antigen retrieval, Autoclave, Staining, Antigen, Pathology, Antibody, Chemistry