Morphogenesis in callus tissue cultures of someMatricaria andAchillea Species
Eva Čellárová, Klára Greláková, Miroslav Repčák, R. Hončariv
Abstract
Eva Čellárová, Klára Greláková, Miroslav Repčák, R. Hončariv
Abstract
In the present paper we deal with the possibility of morphogenesis induction in callus tissue cultures of some representatives of Matricaria and Achillea species. Shoot regeneration from calli of Matricaria chamomilla and M. inodora has been induced by 0.1 mg l -1 kinetin or by combination of 0.5 mg l -1 kinetin and 0.5 mg l -1 NAA added to Murashige-Skoog culture medium. Rhizogenesis took place without any other addition of auxin. In callus tissue cultures of Achillea ptarmica cultivated on Murashige-Skoog medium with 1 mg l -1 2,4-D after a year long cultivation the whole plant has been regenerated without any change of nutrient requirements. In callus tissue of A. nobilis under the same conditions only roots wore regenerated.
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In the present paper we deal with the possibility of morphogenesis induction in callus tissue cultures of some representatives of Matricaria and Achillea species. Shoot regeneration from calli of Matricaria chamomilla and M. inodora has been induced by 0.1 mg l -1 kinetin or by combination of 0.5 mg l -1 kinetin and 0.5 mg l -1 NAA added to Murashige-Skoog culture medium. Rhizogenesis took place without any other addition of auxin. In callus tissue cultures of Achillea ptarmica cultivated on Murashige-Skoog medium with 1 mg l -1 2,4-D after a year long cultivation the whole plant has been regenerated without any change of nutrient requirements. In callus tissue of A. nobilis under the same conditions only roots wore regenerated.
Key concepts: Callus, Kinetin, Botany, Biology, Morphogenesis, Tissue culture, Auxin, Murashige and Skoog medium