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1994•Journal of Medical PrimatologyRequires access

The mitogen‐induced generation of interferon‐gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age‐dependent

Nicole M. van Besouw, Peter H. van der Meide, N.P.M. Bakker

Open publisher page 12 citations

Abstract

An enzyme-linked immunoabsorbent spot (ELISPOT) assay has been developed for measuring the frequency of interferon-gamma (IFN-gamma) producing cells in rhesus monkeys. Aged monkeys revealed, upon mitogenic stimulation, a significantly higher percentage of IFN-gamma secreting peripheral blood mononuclear cells (PBMC) compared to young animals. No correlation was found between the frequency of IFN-gamma producing PBMC and the mitogen-driven proliferation, indicating that in rhesus monkeys no direct correlation exists between these two activation parameters.

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What this paper is about

An enzyme-linked immunoabsorbent spot (ELISPOT) assay has been developed for measuring the frequency of interferon-gamma (IFN-gamma) producing cells in rhesus monkeys. Aged monkeys revealed, upon mitogenic stimulation, a significantly higher percentage of IFN-gamma secreting peripheral blood mononuclear cells (PBMC) compared to young animals. No correlation was found between the frequency of IFN-gamma producing PBMC and the mitogen-driven proliferation, indicating that in rhesus monkeys no direct correlation exists between these two activation parameters.

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Available abstract

An enzyme-linked immunoabsorbent spot (ELISPOT) assay has been developed for measuring the frequency of interferon-gamma (IFN-gamma) producing cells in rhesus monkeys. Aged monkeys revealed, upon mitogenic stimulation, a significantly higher percentage of IFN-gamma secreting peripheral blood mononuclear cells (PBMC) compared to young animals. No correlation was found between the frequency of IFN-gamma producing PBMC and the mitogen-driven proliferation, indicating that in rhesus monkeys no direct correlation exists between these two activation parameters.

Key concepts: ELISPOT, Peripheral blood mononuclear cell, Interferon gamma, Stimulation, Immunology, Peripheral blood, Mitogen-activated protein kinase, Interferon

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The mitogen‐induced generation of interferon‐gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age‐dependent — Research Paper | ScholarLens