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Genetic Transformation of Sugar Beet by Agrobacterium Rhizogenes

Adelina Trifonova, Atanas I. Atanassov

Abstract

The petioles of four in vitro propagated sugarbeet lines were inoculated with Agrobcterium rhizogenes strains. Hairy roots with different frequency were obtained. Root clones resistant to Km were propagated and included in the regeneration experiment. Callus induction and plantlet regeneration from hairy roots was observed in a low frequency. The obtained putative transformants were propagated and rooted in presence of 200mg/l Km. The expression of npt II gene was confirmed in six independent regenerants by dot npt II assay. The integration of marker gene was detected by PCR.

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Key concepts: Plantlet, Agrobacterium, Biology, Callus, Sugar beet, Transformation (genetics), Inoculation, Botany