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The Use of Tannic Acid-Glutaraldehyde in the Study of Elastic and Elastic-Related Fibers

Gerson Cotta-Pereira, F. Guerra Rodrigo, J.F. David-Ferreira

Abstract

Fixation with tannic acid-glutaraldehyde permits distinction of oxytalan, elaunin and elastic fibers in the electron microscope. The results obtained using tannic acid at concentrations of 1.0%, 0.5% and 0.25% in 3% glutaraldehyde were compared. The 0.25% concentration is recommended for studying fine details of connective fibrils and for regular staining of elastin.

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Key concepts: Tannic acid, Glutaraldehyde, Elastin, Staining, Fixation (population genetics), Electron microscope, Materials science, Chemistry