1987•Nucleic Acids ResearchOpen access

Cosmid mapping of the human chorionic gonadotropin β subunit genes by field-inversion gel elect rophoresis

Madge Y. Graham, Tetsuo Otani, Irving Boime, Maynard V. Olson, Georges F. Carle, David Chaplin

Abstract

A cosmid clone containing the entire hCG beta gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCG beta genes in human genomic DNA. A previously uncloned portion of the hCG beta cluster, termed the "gap" region, has been shown not to contain any sequences homologous to the hCG beta cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.

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Key concepts: Cosmid, Biology, Restriction map, Molecular biology, Complementary DNA, Restriction fragment, Genomic library, DNA