Nutritional conditions for the germination of streptomyces galbus 5ME‐13 spores

Abstract The nutritional conditions for the germination of spores of Streptomyces galbus 5ME‐13 were determined under laboratory conditions. The germination of the spores was intiated by the emergence of 1–2 germ tubes after the second hour of incubation and attained its maximum at the sixth hour. This was accompanied by a steady rise in the optical density of the germinating spore suspension. A malt‐extract yeast‐extract medium was found to be the best medium for the germination of the spores. Glycerol as the sole source of carbon was the best supporter for spore germination while, as N source, L‐alanine was preferred. The optimum pH and temperature for spore germination were 7.2 and 30°C, respectively.