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2014•Journal of Liquid Chromatography & Related TechnologiesRequires access

DETERMINATION OF 5-HYDROXYINDOLE-3-ACETIC ACID, DIHYDROXYPHENYLACETIC ACID, AND HOMOVANILLIC ACID IN THE BRAINS OF FREELY MOVING RATS USING MICRODIALYSIS COUPLED WITH HPLC–ECD

Rui Liu, Jin‐Ao Duan, Jianming Guo, Yuping Tang, Dawei Qian

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Abstract

In the present paper, microdialysis (MD) combined with high performance liquid chromatography with electrochemical detection (HPLC–ECD) was applied for the determination of 5-hydroxyindole-3-acetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the hypothalamus of LPS-induced hyperthermia rats. The extracellular neurotransmitters of 5-HIAA, DOPAC, and HVA could be detected increasing significantly in rat brain 150 min after LPS injection. The result showed that dynamic change of neurotransmitters in rat brain could be determined easily by MD coupled with HPLC–ECD. Furthermore, the advantage that fewer animals sacrificed occurred in the microdialysis experiment was confirmed.

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What this paper is about

In the present paper, microdialysis (MD) combined with high performance liquid chromatography with electrochemical detection (HPLC–ECD) was applied for the determination of 5-hydroxyindole-3-acetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the hypothalamus of LPS-induced hyperthermia rats. The extracellular neurotransmitters of 5-HIAA, DOPAC, and HVA could be detected increasing significantly in rat brain 150 min after LPS injection. The result showed that dynamic change of neurotransmitters in rat brain could be determined easily by MD coupled with HPLC–ECD. Furthermore, the advantage that fewer animals sacrificed occurred in the microdialysis experiment was confirmed.

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Available abstract

In the present paper, microdialysis (MD) combined with high performance liquid chromatography with electrochemical detection (HPLC–ECD) was applied for the determination of 5-hydroxyindole-3-acetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the hypothalamus of LPS-induced hyperthermia rats. The extracellular neurotransmitters of 5-HIAA, DOPAC, and HVA could be detected increasing significantly in rat brain 150 min after LPS injection. The result showed that dynamic change of neurotransmitters in rat brain could be determined easily by MD coupled with HPLC–ECD. Furthermore, the advantage that fewer animals sacrificed occurred in the microdialysis experiment was confirmed.

Key concepts: Homovanillic acid, Microdialysis, Chemistry, High-performance liquid chromatography, Chromatography, Acetic acid, 3,4-Dihydroxyphenylacetic acid, Metabolite

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DETERMINATION OF 5-HYDROXYINDOLE-3-ACETIC ACID, DIHYDROXYPHENYLACETIC ACID, AND HOMOVANILLIC ACID IN THE BRAINS OF FREELY MOVING RATS USING MICRODIALYSIS COUPLED WITH HPLC–ECD — Research Paper | ScholarLens