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2004•Journal of Planar Chromatography – Modern TLCRequires access

Densitometric determination of genistin and daidzin in different cultivars of soy (Glycine max)

Zbigniew Janeczko, Jan Krzek, E. Pisulewska, Danuta Sobolewska, Monika Dąbrowska, Urszula Hubicka, Irma Podolak

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Abstract

Conditions have been established for identification and quantification of genistin and daidzin in extracts of different soy cultivars by densitometric thin-layer chromatography. Good resolution of the genistin and daidzin peaks was achieved by chromatographic separation on silica gel F 254 HPTLC plates with chloroform-methanol-water, 23 + 8 + 1 ( v/v ), as mobile phase. Under these analytical conditions there was no interference from other extract components.

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What this paper is about

Conditions have been established for identification and quantification of genistin and daidzin in extracts of different soy cultivars by densitometric thin-layer chromatography. Good resolution of the genistin and daidzin peaks was achieved by chromatographic separation on silica gel F 254 HPTLC plates with chloroform-methanol-water, 23 + 8 + 1 ( v/v ), as mobile phase. Under these analytical conditions there was no interference from other extract components.

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Available abstract

Conditions have been established for identification and quantification of genistin and daidzin in extracts of different soy cultivars by densitometric thin-layer chromatography. Good resolution of the genistin and daidzin peaks was achieved by chromatographic separation on silica gel F 254 HPTLC plates with chloroform-methanol-water, 23 + 8 + 1 ( v/v ), as mobile phase. Under these analytical conditions there was no interference from other extract components.

Key concepts: Genistin, Daidzin, Chromatography, Chemistry, Glycine, Daidzein, Genistein, Biochemistry

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