1976•Nucleic Acids ResearchOpen access

Sequence organization of porcine DNA

Vittorio Enrico Avvedimento, Angela Maria Acquaviva, Stelio Varrone

Abstract

The sequence organization of porcine DNA isolated from thyroid has been analyzed by hydroxylapatite (HAP) chromatography. The reassociation of 0.4 kilobase (Kb) DNA fragments shows, besides the presence of 5% inverted repeat sequences (foldback DNA), that 45% of the genome is represented by high (10%) and intermediate (35%) repetitive components, whereas the remaining 50% is unique sequences. 30% of the unique sequences consists of 1,000 nucleotide fragments interspersed with repetitive elements 400 nucleotides in length. The remaining 20% is longer unique sequences (10,000 nucleotides) apparently not linked to repetitive elements.

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Key concepts: Biology, Repeated sequence, DNA, Nucleotide, Inverted repeat, Nucleic acid sequence, Genomic organization, DNA sequencing