SIMULTANEOUS DETERMINATION OF CREATININE AND URIC ACID IN HUMAN URINE BY CAPILLARY ZONE ELECTROPHORESIS
Li Jia, Xi Chen, Xiaoru Wang
Abstract
Li Jia, Xi Chen, Xiaoru Wang
Abstract
A simple, fast and precise method for the simultaneous determination of creatinine and uric acid in human urine by capillary zone electrophoresis has been developed. The method only requires filtering urine sample and no other pretreatment. The effect of pH of the background buffer on the existent state of creatinine and uric acid was investigated. Photodiode array detection permitted the rapid identification of creatinine and uric acid in the sample analysed. The linear range, detection limit, precision, and recovery of the method was investigated.
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A simple, fast and precise method for the simultaneous determination of creatinine and uric acid in human urine by capillary zone electrophoresis has been developed. The method only requires filtering urine sample and no other pretreatment. The effect of pH of the background buffer on the existent state of creatinine and uric acid was investigated. Photodiode array detection permitted the rapid identification of creatinine and uric acid in the sample analysed. The linear range, detection limit, precision, and recovery of the method was investigated.
Key concepts: Chemistry, Chromatography, Uric acid, Capillary electrophoresis, Creatinine, Urine, Detection limit, Linear range