Nitrogen balance of women consuming cottonseed protein

Abstract and Summary This study investigated the ability of cottonseed protein in liquid formula diets to maintain the nitrogen balance of twelve young women at the level of protein intake usually eaten by adults in the United States. Four levels of defatted cottonseed flour (0, 30, 60, and 100%) and R1–5, a commercially available protein product presumed to be adequate to support nitrogen balance, were used to provide 10 grams of nitrogen. After equilibration each diet was fed for 7 days. Mean daily nitrogen balances were −0.05, S.D. 1.28, +0.76, S.D. 1.62; +0.35, S.D. 2.46; and +1.42, S.D. 1.88 for the 0, 30, 60, and 100% cottonseed diets. The ratios of essential to nonessential amino acids were 0.82, 0.76, 0.67, and 0.59 for the diets from 0 to 100% cottonseed. At the level fed in this study, cottonseed protein adequately maintained nitrogen equilibrium in young adult women.