Centrifugation of Mammalian Cells on Gradients: A New Rotor
Thomas G. Pretlow, Charles W. Boone
Abstract
Thomas G. Pretlow, Charles W. Boone
Abstract
A short-arm rotor increases separation of viable mammalian cells, from mixtures, by low-speed centrifugation; continuous Ficoll density gradients in tissue-culture media are used. We describe the theory and experimental demonstration of the superior separation achieved with this new rotor.
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A short-arm rotor increases separation of viable mammalian cells, from mixtures, by low-speed centrifugation; continuous Ficoll density gradients in tissue-culture media are used. We describe the theory and experimental demonstration of the superior separation achieved with this new rotor.
Key concepts: Ficoll, Centrifugation, Rotor (electric), Differential centrifugation, Separation (statistics), Chromatography, Biophysics, Chemistry