1989•The Tohoku Journal of Experimental MedicineOpen access
A production of Dermatophagoides farinae allergen-specific IgE and Ig(G+M) antibodies by cultured peripheral blood mononuclear cells of patients with extrinsic bronchial asthma.
CHANG-LIANG CHAO, Gen Tamura, S Mue, Tamotsu Takishima
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Abstract
CHAO, C.L., TAMURA, G., MUE, S, and TAKISHIMA, T. A Production of Dermatophagoides Farinae Allergen-Specific IgE and Ig(G+M) Antibodies by Cultured Peripheral Blood Mononuclear Cells of Patients with Extrinsic Bronchial Asthma. Tohoku J. Exp. Med., 1989, 158 (2), 141-148-Using a modified reverse hemolytic plaque assay, we measured in vitro production of Dermatophagoides farinae allergen-specific IgE and Ig(G+M) antibodies by peripheral blood mononuclear cells in 19 patients with extrinsic bronchial asthma. Circulating the allergen-specific IgE was estimated by means of the Phadebas® radioallergosorbent test system. We found a significant difference in the production of the allergen-specific IgE antibody by the cells between asthmatic patients with positive and negative circulating the allergen-specific IgE.
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