2014•Chemical CommunicationsRequires access

A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases

Guojie Zhao, Tianyu Hu, Jun Li, Wei Hua, Hong Shang, Yifu Guan

Abstract

We have developed a rapid and high-throughput assay based on rolling circle amplification, to distinguish individual strand cleavage of DNA duplexes by restriction endonucleases. As an illustration, we analyzed nicking activity of Nb.BbvCI and uneven cleavage of LNA modified DNA by EcoRI. This assay has potential for analyzing protein-DNA interactions.

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Available abstract

Key concepts: Heteroduplex, Cleavage (geology), Restriction enzyme, DNA, Molecular biology, Chemistry, Endonuclease, Single strand