1983•Zeitschrift für Pflanzenernährung und BodenkundeRequires access

Reversed‐Phase High‐Performance Liquid Chromatography of naturally occurring phenolic acids in soils

Ingrid Kögel‐Knabner

Abstract

Abstract A method for the analysis of phenolic acids in plant and soil materials by high‐performance liquid chromatography (HPLC) is described. Separation is by reversed‐phase chromatography, based on differential polarity of the investigated compounds. Optimal separation conditions are achieved by using a concave gradient elution. The HPLC technique offers excellent resolution, reproducibility and an analysis time of 30 Min.

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Available abstract

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Resolution (logic), Reversed-phase chromatography, Gradient elution, Elution, Hydrophilic interaction chromatography