2011•Revista da Sociedade Brasileira de Medicina TropicalOpen access
Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae
José Eduardo Marques Pessanha, Waleska Teixeira Caiaffa, Alzira Batista Cecílio, Felipe Campos de Melo Iani, Simone Costa Araújo, José Carlos Rodrigues Nascimento, Erna Geessien Kroon, Fernando Augusto Proietti, Jorge Rodriguez Arias
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Abstract
INTRODUCTION: To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007. METHODS: Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR. RESULTS: Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes. CONCLUSIONS: Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.
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