Phase microscopic observations in the golgi apparatus in the living cultivated cells.

Phase microscopic and histochernical studies on the alteration of the Golgi apparatus in cultured cells were carried out through the successive stages of mitosis. In non-dividing cells, the cluster of the phase-gray flecks undergoes incessantly considerable changes both in morphology and topography. The mitotic cells exhibit much more drastic alterations of the Golgi apparatus. Fragmentation and dissolution of the phase-gray flecks occur successively during the period from earlier prophase to prometaphase. The cells during from metaphase to mid anaphase contain no recognizable remnant of the phase-gray flecks in their cytoplasm. Then during from late anaphase to early telophase, the Golgi apparatus reappears close to the nucleus in the form of a rosette. This soon comes to be divided into granular flecks, which are scattered to reconstruct the Golgi network of the interphase cell.