Capillary Electrochromatography of Charged Biomolecules with Mixed‐Mode Stationary Phases

Capillary electrochromatography (CEC) is a relatively new hybrid separation technique that combines the selectivity of high performance liquid chromatography with the high efficiency of capillary electrophoresis. Due to its potential, CEC is gathering increasing attention as a powerful separation technique for the complex mixture of biomolecules. However, CEC with traditional silica‐based reversed phase stationary phase (e.g., C18) has some limitations in the separation of ionizable analytes. Recently, the mixed‐mode stationary phase addressed the problems and became an attractive alternative in CEC analysis of biological compounds. This review will introduce several mixed‐mode stationary phases for the CEC separation of charged biomolecules, especially of peptides.