2001•Journal of Clinical MicrobiologyOpen access

Quantification of Human Cytomegalovirus DNA by Real-Time PCR

Elyanne Gault, Yanne Michel, Axelle Dehée, Chahrazed Belabani, Jean‐Claude Nicolas, A. Garbarg‐Chenon

Abstract

A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular gene (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay (P < 0.0001).

About this research paper

What this paper is about

Why it matters

OpenAlex reports 152 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Human cytomegalovirus, Real-time polymerase chain reaction, Cytomegalovirus, Betaherpesvirinae, Virology, Biology, DNA, Viral load