Morphometric Evaluation of the Blood Pressure Related Organs in Nili-Ravi Buffalo (Bubalus Bubalis) With Advancing Age

Forty clinically healthy buffaloes of either sex were divided into two age-groups of equal size i.e., young (12-42 months) and adult (48-96 months). Histological sections were prepared by routine method and stained by H&E. AutoCAD® image analysis software and ocular micrometer were used for the morphometric evaluations. Studies revealed that the adrenal cortex of buffalo was divided into four zones, zona glomerulosa, zona intermedia, zona fasciculate and zona reticularis. The zona fasciculata was recorded as the widest and zona reticularis narrowest zone of the adrenal cortex. The volume of cardiomyocytic nuclei (µm3), volume of renal corpuscles (µm3×103), volume of nuclei of cells of zona glomerulosa (µm3), thickness of the zona glomerulosa, zona intermedia and zona fasciculata of adrenal cortex in adult buffaloes were significantly (P<0.01) higher as compared to their younger counterparts. It is conceivable that the development of blood related parameters showed an increase parallel to the advancing age in order to adjust with the increasing blood pressure due to physiological development process in buffalo.