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2007•Journal of Nippon Medical SchoolOpen access

Xenopus laevis as a Model for the Functional Analysis of Genes Involved in Embryogenesis and Postembryonic Organ Regeneration

Takashi Hasebe, Atsuko Ishizuya‐Oka

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Abstract

The overexpression study is one of the most useful methods for investigating the function of a gene of interest, and this can be easily achieved by using the frog system.Either plasmid DNA digested by meganuclease or synthetic mRNA is injected into Xenopus laevis fertilized eggs to generate transgenic tadpoles or tadpoles transiently overexpressing certain gene(s), respectively (Fig. 1).

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The overexpression study is one of the most useful methods for investigating the function of a gene of interest, and this can be easily achieved by using the frog system.Either plasmid DNA digested by meganuclease or synthetic mRNA is injected into Xenopus laevis fertilized eggs to generate transgenic tadpoles or tadpoles transiently overexpressing certain gene(s), respectively (Fig. 1).

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Available abstract

The overexpression study is one of the most useful methods for investigating the function of a gene of interest, and this can be easily achieved by using the frog system.Either plasmid DNA digested by meganuclease or synthetic mRNA is injected into Xenopus laevis fertilized eggs to generate transgenic tadpoles or tadpoles transiently overexpressing certain gene(s), respectively (Fig. 1).

Key concepts: Xenopus, Regeneration (biology), Embryogenesis, Biology, Cell biology, Gene, Anatomy, Computational biology

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Xenopus laevis as a Model for the Functional Analysis of Genes Involved in Embryogenesis and Postembryonic Organ Regeneration — Research Paper | ScholarLens