1999•Nucleosides and NucleotidesRequires access
Synthesis of Oligodeoxynucleotides Containing 4′-C-Aminoalkyl-thymidines and Their Thermal Stability and Nuclease-Resistance Properties
Yoshihito Ueno, Makiko Kanazaki, Satoshi Shuto, Akira Matsuda
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Abstract
To find the nuclease-resistant oligodeoxynucleotides (ODNs) with natural phosphodiester linkages, we designed and synthesized ODNs containing 4′-C-aminoalkylthymidines (1–4). We found that the ODNs containing 1, 2, 3 or 4 were more resistant to nucleolytic hydrolysis by both snake venom phosphodiesterase (a 3′-exonuclease) and DNase I (an endonuclease) than unmodified ODNs.