Estradiol and Melatonin Effects on Adrenal Cortex of Ovariectomized and Pinealectomized Rats1)

Studies on the effect of pinealectomy (PX) and melatonin on adrenal cortex of ovariectomized and estradiol replaced rats were performed. Neither PX nor melatonin replacement changed corticosterone output by adrenal homogenate, 11 beta-hydroxylase activity, liver corticosterone metabolism or serum corticosterone level, however, melatonin increased adrenal 5 alpha-reductase activity. On the other hand, PX of 14 months duration resulted in an increase in intraadrenal 5 alpha-reductase activity. PX did not modify estradiol effect on rat adrenal cortex, while concomitant estradiol-melatonin administration increased corticosterone output by adrenal homogenate. Stimulatory effect of estradiol on liver corticosterone metabolism was observed only in the presence of pineal gland or exogenous melatonin. In non of experiments, 45 nmole/ml of melatonin added into incubation medium stimulated adrenal 5 alpha-reductase activity. Obtained results cast doubt on a physiological role of melatonin in regulation of adrenocortical secretory activity in ovariectomized rats and suggest that estradiol effect on the adrenal cortex is not mediated by the pineal gland.