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THYROXINE 5′-DEIODINASE ACTIVITY IN BROWN ADIPOSE TISSUE

Jack L. Leonard, SARAH A. MELLEN, REED P. LARSEN

Abstract

Homogenates of rat brown adipose tissue (BAT) and epididymal fat pad (EPI) catalyze the 5'-deiodination of 3,3',5'-triiodothyronine (rT3) in the presence of dithiothreitol. BAT, but not EPI, catalyzes the 5'-deiodination of thyroxinE (T4) generating equal amounts of 3,5,3'-triiodothyronine (T3) and iodide. BAT 5'-deiodinase activity was unaffected by propylthiouracil (PTU) and was elevated in hypothyroid rats, whereas EPI 5'-deiodinase was totally inhibited by PTU and was depressed in hypothyroidism. These results indicate that type II (PTU-insensitive) 5'-iodothyronine deiodinase activity is present in BAT.

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Key concepts: Deiodinase, Internal medicine, Endocrinology, Propylthiouracil, Brown adipose tissue, Iodothyronine deiodinase, Triiodothyronine, Dithiothreitol