2011•Optics in the Life SciencesOpen access

Two-photon Absorbing Probes and Their Use in Two-Photon Fluorescence Microscopy of Cells and Ex Vivo Imaging of Tumors

Ciceron O. Yanez, Carolina D. Andrade, Alma R. Morales, Takeo Urakami, Masanobu Komatsu, Kevin D. Belfield

Abstract

Efficient two-photon (2PA) absorbing dyes and bioconjugates were used in two-photon fluorescence microscopy (2PFM) of cells, tissue sections, and excised tumors. Results show the utility of these dyes in studying biological processes.

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Available abstract

Key concepts: Two-photon excitation microscopy, Fluorescence, Microscopy, Fluorescence microscope, Ex vivo, Multiphoton fluorescence microscope, Photon, Fluorescence-lifetime imaging microscopy