Richard J. Simpson
Abstract
INTRODUCTIONSelective cleavage of Asn-Gly bonds in proteins by hydroxylamine generates a relatively small number of large peptide fragments. This protocol describes a procedure for performing Asn-Gly bond cleavage in solution.
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INTRODUCTIONSelective cleavage of Asn-Gly bonds in proteins by hydroxylamine generates a relatively small number of large peptide fragments. This protocol describes a procedure for performing Asn-Gly bond cleavage in solution.
Key concepts: Hydroxylamine, Cleavage (geology), Peptide bond, Chemistry, Peptide, Stereochemistry, Biochemistry, Biology