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Assay of Serum Vitamin B12 Concentration Using Co57-B12 and Intrinsic Factor

Sheldon P. Rothenberg

Abstract

Based on some observed properties of the B12-I.F. complex, an assay procedure is described for determining serum Vit. B12 levels using isotopically labeled Vit. B12 and intrinsic factor. 83%-112% of crystalline Vit. B12 added to serum was recovered in the assay. Concentrations of Vit. B12 in normal, B12 deficient, and leukemic sera were in the ranges, 104-328 μμg/ml, 0-72 μμg/ml and over 1000 μμg/ml respectively.

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Available abstract

Key concepts: Vitamin B12, Intrinsic factor, Cyanocobalamin, Chemistry, Biochemistry